UHRF1 Antibody
Polyclonal antibody raised in rabbit against UHRF1 (ubiquitin-like with PHD and ring finger domains 1), using a recombinant protein.
| Lot | 45098 |
|---|---|
| Concentration | 1.4 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3 |
| Immunohistochemistry | 1:100 - 1:1,000 | Fig 4 |
| Immunofluorescence | 1:500 - 1:1,000 | Fig 5 |
* Optimal dilutions/concentrations should be determined by the researcher.
Validation Data
Figure 1. UHRF1 ChIP results
ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.
Figure 2. IP results
UHRF1 antibody immunoprecipitates UHRF1 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 μg of UHRF1 antibody (C15410258) 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by western blot with the UHRF1 antibody (C15410258) diluted 1:1,000.
Figure 3. Western blot
Sample: 30 μg of HCT116 whole cell lysate 7.5% SDS PAGE UHRF1 diluted 1:1,000
Figure 4. IHC
UHRF1 antibody detects UHRF1 protein on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. UHRF1 antibody (C15410258) dilution: 1:500.
Figure 5. IFA
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using UHRF1 antibody (Cat. No. C15410258) (Green) at a 1:500 dilution. Red: Alpha-tubulin filaments.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet UHRF1 C15410258 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: UHRF1 Antibody (Hologic Diagenode Cat# C15410258-100 Lot# 45098). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Novel role of UHRF1 in the epigenetic repression of the latent HIV-1. |
DNA methylation enzymes and PRC1 restrict B-cell Epstein-Barr virus oncoprotein expression. |