Universal Plant ChIP-seq kit
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for plant chromatin extraction and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.
- Universal compatiblity with a wide variety of plant species
- Optimized and complete kit for start-to-finish plant ChIP
- Includes plant-specific control primers and control antibody
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C01020014Chromatin EasyShear Kit for Plant
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C15410195H3K27me3 Antibody
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C01010153Auto Universal Plant ChIP-seq kit
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C03010015
IPure kit v2
Characteristics
Successful ChIP-seq experiments for a variety of plants
Arabidopsis
Figure 1. ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.
Poplar
Figure 3. ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.
Tomato
Figure 2. ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.
Maize
Figure 4. ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.
Documents
| Universal Plant ChIP-seq kit MANUAL Manual of the Universal Plant ChIP-seq Kit | Download |
| Cell number estimation plants XLS FILE | Download |
Safety sheets
| Universal Plant ChIP-seq kit SDS GB en | Download |
| Universal Plant ChIP-seq kit SDS US en | Download |
| Universal Plant ChIP-seq kit SDS BE nl | Download |
| Universal Plant ChIP-seq kit SDS FR fr | Download |
| Universal Plant ChIP-seq kit SDS BE fr | Download |
| Universal Plant ChIP-seq kit SDS ES es | Download |
| Universal Plant ChIP-seq kit SDS JP ja | Download |
| Universal Plant ChIP-seq kit SDS DE de | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: Universal Plant ChIP-seq kit (Hologic Diagenode Cat# C01010152). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Temporal modification of H3K9/14ac and H3K4me3 histone marksmediates mechano-responsive gene expression during the accommodationprocess in poplar |
Winter warming post floral initiation delays flowering via bud dormancyactivation and affects yield in a winter annual crop. |
AUXIN RESPONSE FACTOR 16 (StARF16) regulates defense gene StNPR1 upon infection with necrotrophic pathogen in potato. |
GIF1 controls ear inflorescence architecture and floral development byregulating key genes in hormone biosynthesis and meristem determinacy inmaize. |
Chromosomal variations of species revealed by FISH with rDNAs andcentromeric histone H3 variant associated DNAs |
Expression of in the Stem Cell Domain Is Required for ItsFunction in the Control of Floral Meristem Activity in Arabidopsis |
Localization and characterization of Citrus centromeres by combining
half-tetrad analysis and CenH3-associated sequence profiling. |
StE(z)2, a Polycomb group methyltransferase and deposition of H3K27me3 andH3K4me3 regulate the expression of tuberization genes in potato. |
The domesticated transposase ALP2 mediates formation of a novel Polycomb protein complex by direct interaction with MSI1, a core subunit of Polycomb Repressive Complex 2 (PRC2). |
REDOX RESPONSIVE TRANSCRIPTION FACTOR1 (RRFT1) is involved in extracellular ATP regulated Arabidopsis thaliana seedling growth. |
An inferred fitness consequence map of the rice genome. |
Transcription Factor Interplay between LEAFY and APETALA1/CAULIFLOWER during Floral Initiation |
Characterization of the Polycomb-Group Mark H3K27me3 in Unicellular Algae |
Nitric oxide modulates histone acetylation at stress genes by inhibition of histone deacetylases |