V5 Epitope tag Antibody
Polyclonal antibody raised in rabbit against V5 Epitope tag, using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1.0 μg/μl |
| Species reactivity | |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:10,000 - 1:60,000 | |
| Immunohistochemistry | 1:500 - 1:3,000 | |
| Western Blotting | 1:5,000 - 1:10,000 | Fig 3 |
Validation Data
Figure 1. V5 Epitope tag antibody Western blot results
The V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by western blot. The antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full- length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS- PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| IHC Immunohistochemistry Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet V5 Epitope tag C15410270 DATASHEET Datasheet description | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: V5 Epitope tag Antibody (Hologic Diagenode Cat# C15410270 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Release of Immunomodulatory Ebola Virus Glycoprotein-Containing Microvesicles Is Suppressed by Tetherin in a Species-Specific Manner. |